Isolation and Antimicrobial Assessment of Crude Extract from Aspergillus sp. SO12 Isolated from a Marine Source
Ahmed A. Hamed , Mervat G. Hassan, Somaya Sh. Youssef, Mohamed A. Abdelmonem, Dina M. Baraka
The marine environment represents a rich source of microorganisms that have been increasingly explored for their bioactive potential. In this study, we report the isolation of Aspergillus sp. SO12 from a marine source and the subsequent extraction of its crude extract. The bioactive potential of this extract was evaluated through antibacterial assays against various bacterial strains and antifungal assessments against Candida albicans and Aspergillus niger. The isolated strain, Aspergillus sp. SO12, was obtained from a marine sediment sample. Molecular characterization confirmed its identity as a member of the Aspergillus genus, and subsequent cultivation allowed for the production of a crude extract. The extract was obtained using organic solvent. Antibacterial activity of the crude extract was assessed against a panel of clinically relevant bacterial strains, including Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. The extract demonstrated significant antibacterial activity, with notable inhibition zones and minimum inhibitory concentrations (MICs), suggesting the