Adiabatic foot infection is one of the most feared complications of Diabetes mellitus. Many studies have reported on the bacteriology of Diabetic Foot Infections (DFIs) over the past 25 years, but the results have been varied and often contradictory.,Determination the prevalence of beta-lactamase producing bacteria in diabetic foot infections and study the pattern of antibiotic resistance to these isolates, which were received from Mansoura university Hospital by means of antimicrobial susceptibility tests and phenotypic methods. Then confirmed by using Polymerase chain reaction (PCR) to detect Extended-spectrum beta-lactamases (ESBL) genes encodingresistance to beta-lactam antibiotics.
A total of 59 bacterial isolates were obtained from 56 patients with diabetic foot ulcers. The age group of these patients ranged from 40 to 70 years. Gram-positive cocci were more prevalent (50.8%) than gram-neg¬ative bacilli (49.15%). The commonest isolate was Staphylococ¬cus.aureus (33.9%). followed by 15.25 % Pseudomonas.aeruginosa, 11.86 %Proteus.mirabilis. The antibiotic sensitivity profiles of theisolated bac¬teria ,showed that most isolates were resistant to different beta lactams antibiotics .The application of Polymerase chain reaction (PCR) showed that Extended-spectrum beta-lactamases ESBL) producers and the blaTEM, blaSHV, blaCTX-M and blaOXA genes were detected.