Potentiometric determination of terbinafine hydrochloride antifungal drug in pharmaceutical and biological fluids using ion selective electrodes
Tamer Awad Ali ,, Samah M. abdelsabour, Hassan Eldessouky, Gehad G. Mohamed, Yasmin Salah Eldin Elsayed
The target of this study is focused on the determination of terbinafine hydrochloride (TFHC) as antifungal drug. A potentiometric method based on modified screen-printed and modified carbon paste ion-selective electrodes was described for the determination of TFHC in different pharmaceutical and biological fluids. It is based on potentiometric titration of TFHC using modified carbon paste (MCPE) and screen-printed (MSPE) as end point indicator sensors. The influences of the paste composition, different conditioning parameters and foreign ions on the electrodes performance were investigated and response time of the electrodes has been studied. The electrodes showed Nernstian response of 57.83±0.75, 59.06±0.69, 56.99±0.92 and 58.75±1.06 mV decade-1 in the concentration range of 3.8×10-7–1×10-2 and 1×10-7–1×10-2 mol L-1 for MCPE (electrode I and II) and MSPE (electrode III and IV), respectively. The electrodes were found to be usable within the pH range of 3.0–9.0 and 4.0-9.0 exhibited a fast response time (about 9 and 11 s), low detection limit of 3.8×10-7 and 1.0 ×10-7 mol L-1, long lifetime (70, 85 and 152, 166 days) for MCPE (electrode I and II) and MSPE (electrode III and IV), respectively. The electrodes were successfully applied for the determination of TFHC in pharmaceutical preparation and biological fluids (urine and serum). The results obtained applying these potentiometric electrodes are comparable with British pharmacopeia. The method validation parameters were optimized and the method can be applied for routine analysis of TFHC drug.