The current study was conducted to explore the potential of potassium solubilizing for the plant from Gluconite Egyptian ore. Thiobacillus thiooxidans, Rhizobium leguminosarum, Rhizobium rhizogenes and Bacillus megaterium were evaluated for their ability to potassium dissolution from Egyptian Gluconite ore by studying optimization conditions of incubation temperature, incubation time and inoculum size, pH and different carbon and nitrogen as energy sources. Results revealed that dissolution of potassium reached to 30.66, 31,66, 34.44 and 28.87 for Thiobacillus thiooxidans, Rhizobium leguminosarum, Rhizobium rhizogenes and Bacillus megaterium respectively. A model has been suggested which assumes that the potassium was attacked by the reducing bacteria to produce potassium ions of produced organic acids. Kinetically, the leaching process was a zeroorder reaction. In this work applied of microorganism in industrial processes was practically feasible with low cost and friendly environmental process.